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- Writer 이태화
Defense-related proteins and defense mechanisms in Chinese cabbage
- Date/Time : Fri November 22., 2002
- Speaker : 김학용 교수
- Place : Life Science Bldg. #104
- For inquires : Professor Young sook Lee Dept. of Life Science
생명과학과 이영숙 교수 (☎279-2296)
The ability of plants to defend against pathogens, wounding, and salicylic acid depends upon sensitive perception mechanisms. Although genetics approaches have shown that the various resistance genes activate common defense responses, defense-related proteins are not well characterized in plants. We, therefore, examined that salicylic acid and wounding elicit defense-related proteins by using proteomics in Chinese cabbage. Treatments of salicylic acid and wounding elicited PR1a gene and PR1a protein as well. In addition to PR1a protein, we identified several proteins induced by salicylic acid and wounding. Identification of defense-related proteins will provide to understand plant defense mechanisms and signal transduction pathways in Chinese cabbage.
The ability of Pseudomonas syringae pv. tomato (Pst) to elicit the hypersensitive response in non-host plants such as Chinese cabbage is controlled by defense genes and several elicitors. We identified and characterized a putative elicitor from culture medium of the starved Pst. Although the elicitor was identified as a small (Mw 500 or less) organic compound which contains carboxyl group, carbonyl carbon, and hydrocarbon but dose not contain purine, pyrimidine, and aromatic rings, we are still studying to characterize structure of the molecule. Application of the elicitor both onto Chinese cabbage leaf and into protoplasts rapidly induced the accumulation of H2O2, resulting cell death and hypersensitive response. Using further purified elicitor, the nature of cell death, the mechanism of accumulation of H2O2, and Pst-induced defense pathway will be established in Chinese cabbage.