Plant Functional Genomics Using Virus-Induced Gene Silencing

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  • 2014-01-17



Plant Functional Genomics Using Virus-Induced Gene Silencing   


- Date/Time : Fri February 21., 2003


- Speaker : 배현숙 박사

           - Korea Research Institute of Bioscience and Biotechnology

- Place : Life Science Bldg. #104

- For inquires : Professor Inhwan Hwang Dept. of Life Science

            생명과학과 황인환 교수 (☎279-2128)

- Abstract

   Phenomic analysis has been carried out to assess functions of signaling genes and hypothetical genes of Nicotiana benthamiana using TRV-based virus-induced gene silencing (VIGS). VIGS is based on the phenomenon that gene expression is suppressed in a sequence-specific manner by infection with viral vectors carrying host genes (Waterhouse et al., 2001). We have found that gene silencing of various proteasome subunits activates programmed cell death program in plants, providing the first direct evidence that proteasomes play a role in the regulatory program of PCD in plants (Kim et al., 2003). Furthermore, we present evidence that the proteasome regulatory subunit Rpn3 interacts with NtCDPK1 calcium-dependent protein kinase in vivo and in vitro. VIGS of Rpn3 and NtCDPK1 each induced abnormal cell division/expansion, and programmed cell death phenotypes, indicating that NtCDPK1 and NtRpn3 are interacting in a common signal transduction pathway possibly for regulation of cell division/differentiation, and cell death in tobacco (Lee et al., 2003).