Ribocomputing: Leveraging RNA for Computation in the Cell

  • Hit 432
  • Writer 최고관리자
  • 2018-09-04


[2018 Fall Life Sciences & IBB  Seminar]

▶Subject: Ribocomputing: Leveraging RNA for Computation in the Cell

 ▶Speaker:  Prof. Jongmin Kim

 ▶Date: 4:30PM/Sept. 7(Fri.)/ 2018

▶Place: Auditorium(1F), Postech Biotech Center

Synthetic  biology  aims  to  develop  engineering-driven  approaches  to  the  programming  of cellular functions that could yield transformative technologies. Synthetic gene circuits that combine DNA, protein, and RNA components have demonstrated a range of functions such as bistability, oscillation, feedback, and logic capabilities. Despite many advances, technical challenges  remain  for  scaling  up  the  complexity  of  these  networks  due  to  the  limited number of designable, orthogonal, high-performance parts, the empirical and often tedious composition rules, and substantial resource requirements for encoding and operation. Here, we report a  strategy for constructing RNA-only nanodevices to  evaluate complex logic  in living  cells.  Such  ‘ribocomputing’  systems  are  composed  of  de  novo  designed  parts  and operate  via  predictable  and  designable  base-pairing  rules,  allowing  for  effective  in  silico design  of  computing  devices  with  prescribed  configurations  and  functions  in  complex cellular environments. We demonstrate that these ribocomputing devices in Escherichia coli can evaluate two-input logic expressions with dynamic range up to 900-fold and scale them to  four-input  AND,  six-input  OR,  and  a  complex  12-input  logic  expression.  Successful operation of ribocomputing devices based on programmable RNA interactions suggests that systems  employing  the  same  design  principles  could  be  implemented  in  other  host organisms or in extracellular settings.

▶Inquiry: Seung Tae Baek (279-2360)

*This seminar will be given in English.
  Please refrain from taking photos during seminars.*