RNA interference and genome regulation controlling cell fate and devel…

  • Hit 526
  • Writer 최고관리자
  • 2018-08-07


[BK21 Plus Seminar]

▶Subject: RNA interference and genome regulation controlling cell fate and development in Arabidopsis

▶Speaker: Yun Ju Kim, Ph. D. (Center for Plant Aging Research, Institute for Basic Science (IBS))

▶Date: 4:00 PM/Aug. 14(Tue.)/2018

▶Place: Auditorium(1F), Postech Biotech Center

MicroRNA (miRNA) and small interfering RNA (siRNA) belong to 20- to 24- nucleotide-long small non-coding RNAs and act as post-transcriptional and/or transcriptional gene silencers in plants. Despite exciting findings made in the past decade, a mechanistic view of miRNA and siRNA in plant development, defense, and genome stability remains to be further elucidated. We have developed genetic and model systems to investigate functional mechanisms and biological impacts of miRNAs and siRNAs in Arabidopsis. First, we report a cell type-specific genetic screen system, PORI, in which expression of an artificial miRNA (amiRLuc) and its target GFP-Luciferase (GFP-Luc) is temporally overlapped but spatially separated. PORI is a powerful system to study regulatory mechanisms of both miRNA and siRNA through forward and reverse genetic studies. Second, we conducted miRNA profiling of stomatal lineage cells to identify miRNAs and their targets modulating stomatal development. Stomatal cell fate and patterning have been intensively studied in a view of transcriptional control but whether and how miRNA-mediated epigenetic mechanism regulates stomatal development is almost unknown. We provide genetic evidence that developmental stage-specific miRNAs play a crucial role in stomatal development. Together, our studies contribute to better understanding of regulatory sRNA biology in cell fate determination and development in plants.

▶Inquiry: Prof. Il-Doo Hwang (279-2291)

* This seminar will be given in  English.
  Please refrain from taking photos during seminars. *