Extracellular vesicle surface interactions

  • Hit 479
  • Writer 최고관리자
  • 2018-06-04


[BK21 Plus Seminar]

▶Subject: Extracellular vesicle surface interactions

▶Speaker:  Prof. Edit I Buzás (Semmelweis University, Budapest, Hungary)

▶Date: 2:00 PM/Jun,  8(Fri.)/2018

▶Place: : Auditorium(1F), Postech Biotech Center

Extracellular vesicles are phospholipid bilayer enclosed structures that establish important interactions with both the plasma membrane of cells and molecules of the extracellular matrix. Extracellular vesicle surface interactions may impact, among others, vesicular stability, immune recognition and cellular uptake of vesicles. Proteomic analysis of extracellular vesicles isolated from complex matrices such as blood plasma show substantial amounts of plasma proteins co-isolated with extracellular vesicles. Moreover, we found that extracellular vesicle preparations isolated by currently available methods fail to purify extracellular vesicles from blood plasma without lipoproteins such as LDL. In vitro mixing of LDL with either microvesicles or exosomes resulted in a striking attachment of LDL onto the surface of isolated vesicles. LDL binding onto the surface of extracellular vesicles interfered with detection of conventional extracellular vesicle surface markers by flow cytometry. We have also demonstrated ApoB100 positive Triton sensitive (vesicular) events in the pericardial fluid of patients with coronary artery bypass surgery. In addition, we found that genotoxic stress of cells results in the release of exosomes with DNA attached onto their exofacial surface. In contrast, cell activation or apoptosis induction failed to induce such exosomal release of DNA. Using an optical biosensor, we demonstrated that DNA attached onto the exofacial surface of exosomes could mediate exosomal binding to fibronectin. Our data suggest that extracellular vesicle surface interactions are not restricted to those with proteins but may also include association of extracellular vesicles with lipoproteins and nucleic acids.

▶Inquiry: Prof. Yong Song Gho (279-2345)

* This seminar will be given in English.
Please refrain from taking photos during seminars. *