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- Writer 이태화
Functional Analysis of Regulatorry Genes Involved in
Arabidopsis Leaf Senescence
- Date/Time : Tue September 16., 2003
- Speaker : Prof. Vicky B-Wollaston,
- Horticulture Research International
- Place : Life Science Bldg. #104
- For inquires : Professor Hong Gil Nam Dept. of Life Science
생명과학과 남홍길 교수 (☎279-2111)
- Abstract -
Senescence in leaves represents the final stage of development and is rapidly followed by death and abscission. The efficient transport of nutrients from leaves to seeds in developmental senescence is important for plant growth and to maximise seed yield. Post-harvest senescence, in green vegetable crops and in flowers, affects shelf life and leads to products with reduced nutrient quality and appearance. Therefore, identification of key regulatory steps involved in the control of senescence will provide plant breeders with tools for the generation of crops with improved yield, stress-resistance and also post-harvest quality.
Leaf senescence is a programmed event responding to a wide range of external and internal signals. It requires de novo gene expression and protein synthesis and is controlled in a tightly regulated manner. Many different genes show enhanced expression during senescence and elucidation of the roles of these genes is throwing light on the mechanisms that occur during the senescence process. Elucidation of the genes that control senescence has been complicated by the complex combination of signalling pathways that appear to be involved in senescence. Cross talk exists between senescence and stress or pathogen responses and also hormonal and nutrient signals are implicated in the control of senescence.
We are using Arabidopsis as a model, to study the genes involved with the control of leaf senescence. Microarray analysis is being used to identify senescence-enhanced genes and also to analyse the downstream effects of knock out mutations in senescence enhanced regulatory factors. Results from these experiments will help to elucidate the signalling pathways involved in senescence and should pinpoint key controlling steps in the process. We are also studying gene expression changes during post harvest senescence in petals and broccoli florets and comparing gene expression patterns between developmental and post harvest senescence.