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Comparative Interactomes of VRK1 and VRK3 with Their Distinct Roles in…

  • 저널명
    • 담당교수Kyong Tai Kim
    • 조회829
    • 작성자최고관리자
    • 2017-09-28


    Vaccinia-related kinase 1 (VRK1) and VRK3 are members of the VRK family of serine/threonine kinases and are principally
    localized in the nucleus. Despite the crucial roles of VRK1/VRK3 in physiology and disease, the molecular and functional
    interactions of VRK1/VRK3 are poorly understood. Here, weidentified over 200 unreported VRK1/VRK3-interacting candidate
    proteins by affinity purification and LC-MS/MS. Thenetworks of VRK1 and VRK3 interactomes were found to be associated with important biological processes such as the cell cycle, DNA repair, chromatin assembly, and RNA processing.
    Interactions of interacting proteins with VRK1/VRK3 wereconfirmed by biochemical assays. We also found that phosphorylations of XRCC5 were regulated by both VRK1/VRK3, and that of CCNB1 was regulated by VRK3. In liver cancer
    cells and tissues, VRK1/VRK3 were highly upregulated and its depletion affected cell cycle progression in the different phases.
    VRK3 seemed to affect S phase progression and G2 or Mphase entry and exit, whereas VRK1 affects G1/S transition in
    the liver cancer, which could be explained by different interacting candidate proteins. Thus, this study not only provides a
    resource for investigating the unidentified functions of VRK1/VRK3, but also an insight into the regulatory roles of VRK1/VRK3 in biological processes.