POSTECH 생명과학과
Seminar
Seminar

Architecture mapping of mitochondrial protein complexes by proximity l…

  • Hit 261
  • Writer 최고관리자
  • 2018-04-30

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▶Subject: Architecture mapping of mitochondrial protein complexes by proximity labeling in live cells

▶Speaker: Prof. Hyun-Woo Rhee (Department of Chemistry, Seoul National University)

▶Date: 4:30PM/May. 4(Fri.)/2018

▶Abctract
Proximity labeling is an in situ protein biotinylation reaction by enzymatically generated reactive biotin-species which have a short lifetime (less than 1 ms) in aqueous solution. Recently, proximity labeling has shown remarkable new findings in biological research fields such as sub-organelle proteome mapping and in vivo interactome identification which have not been easily obtained by conventional methods. Our group recently developed an improved proximity labeling method (Spot-ID) by mass-spec detection of the biotin-labeled proteins which are generated by engineered ascorbate peroxidase (APEX) or promiscuous biotin ligase (pBirA). This method allows the direct identification of the labeled protein without false positive findings and the structural identification of the labeled protein in live cells. Using this method, we could map in vivo proteomic architecture of the inner mitochondrial membrane (IMM) whose membrane topology has not been fully characterized yet. Furthermore, I will introduce a new approach to perform proximity crosslinking by utilization of a simple photo-crosslinking reaction in live cells. Using this method, we could identify physically interacting molecular partner(s) related to piRNA biogenesis on the mitochondria in live cells

▶Inquiry: Prof. Joo-Yeon Yoo (279-2346)

* This seminar will be given in English.
Please refrain from taking photos during seminars. *

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